Learning Exercise

Quantitative Polymerase Chain Reaction

This module introduces the math behind quantitative PCR reactions and follows a module on PCR. The PCR reaction is best described as an exponential growth function.
Course: Molecular Biology
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Shockwave animations of PCR, Southern Blotting, Cycle Sequencing, Ac/Ds transposition and three case studies using DNA... see more

Exercise

This activity includes two files. A powerpoint tutorial on how to create a spreadsheet that models the quantitative PCR reaction. Also an excel spreadsheet containing the final graphs.

Topics

Quantitative Polymerase Chain Reaction

Learning Objectives

The goal of this module is to introduce the basic mathematics that describes exponential growth. Students should become familiar with using spread sheets to calculate rate of growth.

Assessment

Pre and post-assessment tools are embedded in the powerpoint file.