The Polymerase Chain Reaction in the Diagnosis of Disease
Each of the DNA sequences below was derived from a different bacterium A - E.
1. What four nucleotide primers would allow replication of the complete DNA
sequence from both strands of bacterium A?
2. To which of the bacteria would each of the following probes bind? To which
3. Design a four-nucleotide probe that will recognize only bacterium E.
4. What is the difference between a probe and a primer?
5. Explain how pcr can be used to identify a pathogen within a specimen from a
patient. Why does pcr have such high sensitivity and specificity as a diagnostic
Introduction to concepts of sensitivity and specificity.
diagnosis of infectious disease
To describe the process of pcr.
To discuss the advantages and disadvantages of pcr as a diagnostic tool.