This site animates the microarray procedure using transcription from the Herpes Simplex virus genome in HeLa cells as an example.
To help students understand how microarrays are used to gain knowledge of gene expression
Target Student Population:
Upper level undergraduates
Prerequisite Knowledge or Skills:
basic understanding of DNA and gene expression
Type of Material:
To help illustrate microarray procedure either in class or as out-of-class review
Evaluation and Observation
High quality animation that illustrates the production of microarrays, sample preparation, and analysis of microarray data.
Animation does a good job of showing the actual steps, and also zooms in on what is occurring at a molecular level on the microarray.
The appropriate labels and writing are included in an uncluttered manner
The final microarray shows green spots for RNA expressed in uninfected cells.
How does HSV RNA get expressed there? Either the microarray is showing HeLa cellgenes not HSV or this was another experiment of HSV at different time points.
A clearer, or zoomed in, picture of the microarray would help the students see the subtle differences in color between spots.
Potential Effectiveness as a Teaching Tool
Basic principles are clearly and effectively presented
Useful as an animation in lecture, where the instructor can explain what is happening at each step.
The animation is direct and to the point and only requires a short time to run
Could develop assignments based upon the animation.
There is no way to pause the animation
There is no interactivity or problem solving component to check for understanding. While assignments could be developed, there are no assignments or links to assignments on the site. It is not clear what the students should learn from the animation.
The interpretation of the results is incomplete. It would help if a few of the spots were identified, and the significance of the results explained (or better yet ask the students to explain the significance).
There is little explanation of why someone would want to do a microarray,
i.e. why do we care which genes are expressed at different times?
Ease of Use for Both Students and Faculty
The animation loaded quickly and was very easy to use.
The replay button at the end allows for reviewing the process
It would be nice to be able to pause the animation for use in lecture, or to give students an opportunity to answer questions.
Comments from Author:
This site was intended for a specific purpose for my own web site.