This tutorial provides information on using various combinations of restriction enzymes to determine the sequence of 3.0kb piece of DNA in a pBluescript plasmid. It explains how to map single cuttes and multiple cutters by examining restriction fragments in order to determine the linear sequence of fragments along a larger piece of DNA. In includes solutions to the problem, and some strategy on restriction mapping.
To understand how to use various restriction digests to determine the linear restriction may showing the relative position of various restriction sites.
Target Student Population:
Prerequisite Knowledge or Skills:
Basic molecular biology
Type of Material:
Out of class practice assignment.
Evaluation and Observation
A realistic problem, uses real enzymes and plasmids.
Gives some good hints in how to interpret restriction digests.
Lets students see what restriction digest gels look like for the left-side and right-side enzyme digests.
Mostly text based, no interesting story worked into the cloning strategy, i.e. why do this?
Could use more background material explaining the process.
Figure 1 is almost impossible to read.
The interactive piece of the tutorial, where you chose two different enzymes and are supposed to view the results, is not functional.
Potential Effectiveness as a Teaching Tool
Interactive problem solving tutorial.
The information is somewhat useful, but the same information is available with better quailty in many lab manuals and textbooks.
Not very open-ended or inquiry-based.
Only one solution, a more animated program could shuffle the scenario, allowing students to solve multiple problems with different answers.
Similar to static textbook problems.
Ease of Use for Both Students and Faculty
Loaded quickly and all on one page.
The problems with readability, and additionally the fact that the only interactive component of the module does not function, leave little to recommend this tutorial.
Other Issues and Comments:
I would suggest the author do a major revision of this site or remove it from access, as it is not functional in its current state.