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Complementary and alternative medicine (CAM) has become increasingly popular and reported for countless benefits in biomedical health care systems. The study assessed the potential impact of The Trivedi Effect® (biofield energy) on Shigella sonnei for changes in antimicrobial sensitivity, biochemical study, and biotype number using MicroScan Walk-Away® system. The cells were obtained from MicroBioLogics Inc., USA bearing the American Type Culture Collection (ATCC 9290) number, and divided into two groups, Group (Gr.) I: control and Gr. II: treated. Gr. II was subjected to Mr. Trivedi’s biofield energy treatment and further subdivided into two sub-groups, Gr. IIA and Gr. IIB. Gr. IIA was analyzed on day 10, while Gr. IIB was stored and analyzed on day 160 (Study I). The Gr. IIB sample was retreated on day 160 (Study II), and was divided into three separate tubes as first, second and third tube, which were analyzed on day 5, 10 and 15, respectively. Results showed that 35% (7 out of 20) antimicrobials were reported with improved sensitivity profile. Moreover, the minimum inhibitory concentration study showed that 56.25% (18 out of 32) tested antimicrobials were reported with decreased concentration by two to four-fold as compared with the control after biofield treatment. The effect was further analyzed and sustained in the biochemical study, where 57.57% (19 out of 33) tested biochemicals showed altered reaction pattern as compared with the control. The biotype study showed an alteration in the biotype number in all the experimental treated groups as compared to the control. Encouraging results suggests that bioenergy healing treatment as an integrative medicine against S. sonnei would be a better and safe treatment approach in near future.

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