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A simple classroom lab which uses the enzyme Lactase to demonstrate the specificity of enzymes to a substrate (in this case, Lactose). Also, demonstrates how heat can denature enzymes. Glucose test strips are used to test for the presence of this sugar in milk, and a simple sugar solution, before and after lactase has been added.
enzyme, chemical reaction, lactase, lab, biology, denature, lactose
Great lab, I am planning on using this in the classroom in the spring
Time spent reviewing site:
4 years ago
I would definitely be interested in using this lab as an adjunct to teaching enzymes in the macromolecules unit in Biology. It also reviews monosaccharides and disaccharides as well. Good example of scientific method, how a ‘control’ is made, and how enzymes are specific for their substrates.