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A virtual laboratory which allows users to analyse intracellular signalling pathways. The programme allows the student to stimulate cells for different periods of time and analyse phosphorylation/activation of kinases in the signalling pathways, using SDS-PAGE and immunoblotting.Use of different cell types (dominant-negative...
A virtual laboratory which allows users to analyse intracellular signalling pathways. The programme allows the student to stimulate cells for different periods of time and analyse phosphorylation/activation of kinases in the signalling pathways, using SDS-PAGE and immunoblotting.
Use of different cell types (dominant-negative mutants) and pull-down assays allows them to derive the hierarchy in the signalling pathways. The programme first introduces the theory behind the techniques. It then takes the student through a series of guided questions, which they answer by going to the virtual laboratory to devise and interpret their own experiments. Results from the experiments (gels and blots) can be saved to produce laboratory reports.
The programme is an installer which places all the files required to run the lab, plus the lab manual (pdf) and a model lab-report (doc) onto the hard-drive in a folder 'BioUKoer - Open University S377 Molecular and cell biology’. The laboratory programme is an executable file, written in C++.
It can also be downloaded to, installed and run from a memory stick, allowing those with no admin rights to still access the lab.