The present study was aimed to investigate the potential role of Consciousness Energy Healing (The Trivedi Effect®) based DMEM medium on the level of alkaline phosphatase enzyme (ALP) in Ishikawa cells. The test item (DMEM medium) was divided into two parts, one part received Biofield Energy Treatment by a renowned Biofield Energy Healer, Alice Branton and was categorized as the Biofield Energy Treated DMEM group, while another part did not receive any sort of treatment and coded as the untreated DMEM group. The cell viability using MTT assay showed a significantly improved cell viability upto 98% in the test item group, which suggested a safe and nontoxic profile of the test item. Estrogenic potential using estimation of ALP level showed a significantly increased by 30.8% in the Biofield Energy Treated group as compared with the untreated DMEM group. The Biofield Energy Treated DMEM showed a significant role in the growth of Ishikawa cells along with an improved ALP level that can be utilized significantly in the promotion and maintenance of estrogen level. It is concluded that Biofield Energy Healing Treatment showed a significant improved ALP level, which can be used in different menstrual and estrogenic disorders like hypophosphatasia, osteoporosis, malnutrition, hypothyroidism, magnesium deficiency, heart surgery, chronic myelogenous leukemia, enteritis in children, pernicious anemia, bacterial infection and intrauterine infection is a leading cause of pelvic inflammatory disease, subfertility, infertility, endometritis, early pregnancy loss, fetal defects, and preterm birth.