Learning Exercise

Polymerase Chain Reaction

This module introduces the math behind PCR reactions and precedes a second module on quantitative PCR. The PCR reaction is best described as an exponential growth function.
Course: Molecular Biology
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Shockwave animations of PCR, Southern Blotting, Cycle Sequencing, Ac/Ds transposition and three case studies using DNA... see more

Exercise

There are two files. The first is a powerpoint tutorial that students can use to generate a spreadsheet simulating PCR amplification. The second is an excel spreadsheet with the finished product.

Topics

Polymerase Chain Reaction

Learning Objectives

The goal of this module is to introduce the basic mathematics that describes exponential growth. Students should become familiar with using spread sheets to calculate rate of growth.

Assessment

There are embedded pre and post-assessment tools in the powerpoint.