Electroporation and marker exchange of Pseudomonas syringae pv. syringae.
This resource is a laboratory exercise that will introduce students to the technique of electroporation. More specifically, this exercise uses the the technique of electroporation for the introduction of plasmids into P. syringae pv. syringae. Students will learn how electroporation parameters affect transformation efficiency, and how electroporation can be used to introduce mutated genes into the genomes of bacteria via homologous recombination. This laboratory exercise is designed to be adapted to the instructor's available equipment as well as the goals of the instructor. It is divided into three sections with separate protocols, and the instructor can choose which sections will be most effective in their respective courses. They are listed in order of difficulty.