Material Detail

Transformation of E. coli Using Green Fluorescent Protein

In this activity related to plant biotechnology, learners transform a strain of E. coli using green fluorescent protein from a bioluminescent jellies. This lab will help learners understand recombinant DNA techniques, including the transformation procedure using the heat shock method, the uses of marker or reporter genes in molecular biology experiments and how to screen for a gene of interest, how DNA can be transferred to another organism and therefore change the observable characteristics of that organism, and how to calculate transformation efficiency. Learners will also become familiar with sterile technique and decontamination procedures to safely handle bacteria. Although the E. coli strain used in the experiment is non-pathogenic, it's important to emphasize proper safety... Show More

Keywords:: Social Sciences, Viruses and Bacteria, NSDL, Ecology, Green Fluorescent Protein, Cells, Physics, Plasmid, Number and Operations, Genetic Engineering, Chemistry, Chemistry of Life, General, DNA, Light and Optics, Heredity and Genetics, Science and Society, Agriculture and Biotechnology, Antibiotic, Bioengineering/Biomedical Engineering, Life Science, Physical Science, Molecular Biology, Communicating Results, Cell Structure and Function, Mathematics, Anthropology, Reporter Gene, Marker Gene, Engineering, Cell Division, Education, Biological Science, Jellyfish, Conducting Investigations, Animals, Health/Medicine, Data Representation, Data Analysis, Forestry and Agriculture, Materials Science, Genes, Recombinant DNA, Data Collection, Fluoresce, Gathering Data, Jellies, The Scientific Process, General Science, Technology, DNA Structure and Function, Genetic Transformation, Data Analysis and Probability, Bacteria, Agriculture, Formulating Explanations