Bone disorder like osteoporosis is an important risk factor for fragility fractures. The main objective of this study was to find the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items (viz. vitamin D3 and DMEM), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Cathryn Dawn Nykvist and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Alkaline phosphatase (ALP), collagen, and bone mineralization activities were performed to assess bone health in human bone osteosarcoma cells (MG-63). The test samples were found as safe in the tested concentrations by MTT assay. ALP was significantly increased by 88.68% and 166.1% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item, respectively at 10µg/mL than the UT-DMEM + UT-Test item group. Moreover, the ALP level was significantly elevated by 51.99% and 58.32% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 100µg/mL compared to the untreated group. Collagen was significantly increased by 135.60%, 149.72%, and 95.90% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 100µg/mL compared to the untreated group. Further, the collagen level was significantly increased by 83.43% in the BT-DMEM + BT-Test item group at 10µg/mL, while 43.00% and 82.81% in the UT-DMEM + BT-Test item and BT-DMEM + BT-Test item groups, respectively at 50µg/mL compared to the untreated group. Besides, the percent of bone mineralization was distinctly increased by 103.28%, 120.78%, and 84.26% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 1µg/mL compared to the untreated group. Further, percent of bone mineralization was distinctly increased by 104.41% and 81.71% in the UT-DMEM + BT-Test item and BT-DMEM + UT-Test item group at 0.1µg/mL, respectively compared to the untreated group. Overall, the Biofield Energy Treated vitamin D3 was significantly improved the bone health parameters and it could be a powerful alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone related problems including rickets, osteomalacia, osteoporosis, osteogenesis imperfecta, Paget’s disease, bone and joint pain, low bone density, bone fractures, osteoma, chondrodystrophia fetalis, stress management and prevention, autoimmune and inflammatory diseases, and anti-aging by improving overall health.
