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Lactase Enzyme Lab


Lactase Enzyme Lab

Logo for Lactase Enzyme Lab
A simple classroom lab which uses the enzyme Lactase to demonstrate the specificity of enzymes to a substrate (in this case, Lactose).  Also, demonstrates how heat can denature enzymes.  Glucose test strips are used to test for the presence of this sugar in milk, and a simple sugar solution, before and after lactase has been added.
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Material Type: Assignment
Technical Format: HTML/Text
Date Added to MERLOT: September 04, 2009
Date Modified in MERLOT: September 04, 2016
Keywords: enzyme, chemical reaction, lactase, lab, lactose, biology, denature


  • Reviewed by members of Editorial board for inclusion in MERLOT.
    Editor Review
    Very good quality; in queue to be peer reviewed
    avg: 5 rating
  • User review 4.25 average rating
  • User Rating: 4.25 user rating
  • Discussion (2 Comments)
  • Learning Exercises (none)
  • Bookmark Collections (1)
  • Course ePortfolios (none)
  • Accessibility Info (none)

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Primary Audience: High School, College General Ed, College Lower Division
Mobile Compatibility: Not specified at this time
Technical Requirements:
Language: English
Cost Involved: no
Source Code Available: unsure
Accessibility Information Available: unsure
Creative Commons: unsure


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Discussion for Lactase Enzyme Lab

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Avatar for Anil Kapoor
4 years ago

Anil Kapoor (Faculty)

Great lab, I am planning on using this in the classroom in the spring

Time spent reviewing site: 35 min

Avatar for Shirley Baker
7 years ago

Shirley Baker (Student)

I would definitely be interested in using this lab as an adjunct to teaching enzymes in the macromolecules unit in Biology. It also reviews monosaccharides and disaccharides as well. Good example of scientific method, how a ‘control’ is made, and how enzymes are specific for their substrates.