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PCR polymerase chain reaction

PCR polymerase chain reaction

A common method of amplifying target sequences of DNA in vitro by polymerase chain reaction (PCR) is described in this simplified yet accurate animation. The cycles of amplification are described verbally as well as visually including the melting of DNA strands, the adherence of primers, and construction of the target sequence from free nucleotides by Taq polymerase. The cycle is repeated to demonstrate the power of PCR to exponentially amplify target sequences of DNA to the exclusion of non-target sequences.



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