Chromium trioxide (CrO3) has many industrial applications for the manufacturing of aerospace parts, synthetic rubies, stripping agent, etc. but it is very hygroscopic, toxic and powerful oxidizing material. The objective of this study was to evaluate the influence of the Trivedi Effect®-Consciousness Energy Healing Treatment on the physicochemical and thermal properties of CrO3 using modern analytical techniques. The sample was divided into two parts, one part of the test sample was considered as the control sample (no Biofield Treatment was provided), while another portion received the Biofield Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak and termed as the treated sample. The particle size values of the treated CrO3 were increased by 2.39%, 4.52%, 4.42%, and 4.15% at d10, d50, d90, and D(4,3), respectively compared to the control sample. Thus, the specific surface area of the treated CrO3 was decreased by 4.27% compared with the control sample. The PXRD peak intensities of the treated CrO3 were significantly altered ranging from -73.48% to 212.5% compared with the control sample. Similarly, the crystallite sizes of the treated sample were significantly altered ranging from -38.7% to 167.39% compared to the control sample. However, the average crystallite size of the treated sample was increased by 4.25% compared with the control sample. The latent heat of fusion of the treated sample was significantly increased by 556.45% compared with the control sample. The Trivedi Effect®-Consciousness Energy Healing Treatment might produce a new polymorphic form of CrO3 which would be better powder flowability and lower solubility; hence it will show the lower absorption and toxicity on inhalation, ingestion, contact to skin and eye, chronic exposure, and aggravation of pre-existing conditions. The Biofield Energy Treated CrO3 sample would be very useful for the ceramic industry manufacturing the synthetic rubies, stripping agent of anodic coatings, aerospace parts, etc.