The aim of the present study was to evaluate the cellular (CD4+, CD8+, and CD4+ CD25+) and humoral immune (IgA, IgE, IgG, and IgM) responses of Consciousness Energy Healing Treatment (the Trivedi Effect®) based novel test formulation in unpredictable chronic stress (UCS) in male Sprague Dawley (SD) rat model. A test formulation contained minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and the animals received Biofield Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The experimental results showed Biofield Treatment per se to the rats (G6) increased the IgM and IgE levels by 133.33% (p ≤ 0.001) and 631.25%, respectively as compared to the disease control group (G2). However, 15-days pre-treatment of Biofield Energy Treated test formulation (G7) significantly improved the level of IgM and IgE by 200.98% (p ≤ 0.001) and 213.54% respectively, as compared to the G2. 15-days pre-treatment of the Biofield Energy Treated test formulation to the Biofield Energy Treated rats (G8) showed an increased IgM and IgE levels by 173.04% (p ≤ 0.001) and 355.21%, respectively as compared to the G2. The untreated test formulation to the Biofield Energy Treated rats (G9) also showed an increased IgM and IgE level by 212.75% (p ≤ 0.001) and 247.92%, respectively as compared the G2. Besides, cellular immune data showed that CD4+ level was significantly increased in the G6, G8, and G9 groups by 27.39%, 18.80%, and 25.79%, respectively as compared with the G4. CD8+ level was significantly improved by 18.42%, 43.93%, 27.43%, 43.02%, and 37.75% in the G5 (Biofield Energy Treated test formulation), G6, G7, G8, and G9 groups, respectively as compared with the untreated group, G4. Overall, the data suggested significance effect of Biofield Energy per se along with preventive measure on the animal with respect to various immune-related disorders. Therefore, the results showed the significant improve the human body immune responses, enhance resistance towards diseases, allergies, lethargic conditions, energy booster action, and its various immune deficiency diseases along with its associated complications / symptoms can be preventive using Biofield Energy Treatment per se and / or Biofield Energy Treated Test formulation groups.
