This present study evaluated the impact of Consciousness Energy Healing/Blessing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats, fed with Vitamin D3 Deficiency Diet (VDD) for the estimation of mechanistic biomarkers such as endothelin-1 and nitric oxide (NO) in kidney, lungs, and artery tissues. A novel proprietary test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The novel test formulation was divided into two parts; one part was defined as untreated test formulation, while the other of test formulation and three group of animals received Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Kidney endothelin-1 level was significantly increased by 33.9%, 94.6% (p<0.001), 19.2%, and 14.6% in Biofield Energy Treated/Blessed test formulation group (G5), Biofield Energy Treatment per se (G6), 15 days pre-treatment of Biofield Energy Treated test formulation (G7), and untreated test formulation to the Biofield Energy Treated animals (G9) groups, respectively as compared with the untreated group (G4); while 66.9% (p<0.001) in the G6 group as compared to the G2 group. Similarly, the level of NO in kidney was significant increased by 10.6% and 10.4% in the G7 and G8 groups, respectively as compared with the G4. However, lungs endothelin-1 was reported to be increased by 62.7%, 18.8%, 26.9%, and 35.5% in the G6, G7, G8, and G9 groups, respectively as compared to the G2. Similarly, the lungs NO level was significantly increased by 18.4% and 54.3% in the G6 and G7 groups, respectively, as compared with the G4. The level of endothelin-1 in artery was increased by 13.6%, 16.7%, and 28.2% in G5, G6, and G7 groups, respectively as compared with the G2 group. Similarly, the level of No in artery was increased by 107.4%, 65.4%, 71.9%, 59.7%, and 37.1% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. Altogether, the Biofield Treated test formulation and Biofield Energy Treatment per se significantly used as an effective approach to improve the functioning of endothelin-1 and NO in kidney, lungs and artery that can be used against many disorders such as Alzheimer’s disease, dementias, brain cancer, epilepsy and other seizure disorders, mental disorders, and Parkinson’s. Overall, the results showed the significant slowdown the disease progression and disease related all other complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease group.
