Sulfamethoxazole is an antibiotic used for the treatment of infections caused by bacteria. The experiment was performed to evaluate the impact of the Trivedi Effect® on the structural properties and the isotopic abundance ratio of sulfamethoxazole using LC-MS and GC-MS analytical techniques. Sulfamethoxazole sample was divided into two parts, one part of sulfamethoxazole was considered as a control sample, while the other part only received the Consciousness Energy Healing Treatment remotely by a wellknown Spiritual Energy Healer, Mr. Mahendra Kumar Trivedi and termed as a treated sample. In both the samples, LC-MS spectra showed at retention time (Rt) 2.51 minutes, that exposed the mass of the deprotonated molecular ion peak at m/z 252 [M-H]- (calculated for C10H10N3O3S-, 252.04). The peak area of the treated sulfamethoxazole was significantly increased by 55.17% than untreated test item. The LC-MS-based isotopic abundance ratio of PM+1/PM in the Biofield Treated/Blessed sulfamethoxazole was significantly decreased by 55.57% than untreated. Similarly, the GC-MS peak area% of the treated sulfamethoxazole was significantly increased by 12.96% than untreated test item. The GC-MS-based isotopic abundance ratio of PM+1/PM and PM+2/PM in the Biofield Treated/Blessed sulfamethoxazole was significantly decreased by 15.86% and 8.8%, respectively than untreated test item. The isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O or 33S/32S) and PM+2/PM (18O/16O or 34S/32S) in the treated sulfamethoxazole were significantly reduced than untreated test item. Thus, 13C, 2H, 15N, 17O, 18O, 33S, and 34S contributions from (C10H11N3O3S)+ to m/z 254 and 255 in the treated sample were significantly reduced than untreated test item. The reduced isotopic abundance ratios would highly influence the atomic bond vibration, chemical bond strength, and the stability of treated sulfamethoxazole. It can be envisaged that the changes in peak area%, isotopic abundance, and mass peak intensities could be due to changes in nuclei, possibly through the interference of neutrino particles via the Trivedi Effect®. The new form of sulfamethoxazole would be more efficacious pharmaceutical formulations that might offer better solubility, dissolution, absorption, bioavailability, and better therapeutic response against urinary tract infections, tuberculosis, diarrhoea, ear infections, bronchitis, shigellosis, and Pneumocystis jiroveci pneumonia, etc.
