The objective of the present study was to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated Test formulation (TI) containing minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on ATP levels in human osteoblast (MG-63, Osteosarcoma) and mouse myoblast (C2C12) cell lines. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation (UT), while the other portion of the test formulation received Biofield Energy Healing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing Treatment and known as Biofield Energy Treated (BT) test items. MTT results showed that the test formulation in various concentrations was found as safe and nontoxic, with the viability range from 82% to 159%. However, the level of adenosine tri phosphate (ATP) in MG63 cells was increased by 146.9% (at 0.001 μg/mL) in the UT-DMEM+BT-TI group as compared with the UT-DMEM+UTTI group. However, 79.6%, 111.9%, and 180.7% increased the level of ATP was reported at 0.1, 1, and 10 μg/mL, respectively in the BTDMEM+UT-TI group, while 456.9%, 91.5%, and 123.4% increased ATP level was reported at 0.001, 0.01, and 0.1 μg/mL, respectively in the BT-DMEM+BT-TI group as compared with the UT-DMEM+UT-TI group in MG63 cells. Similarly, in the C2C12 cell lines were also reported with significant increased the ATP level by 178.7% at 1 μg/mL in the UT-DMEM+BT-TI group compared to the UTDMEM+UT-TI group. Further, the ATP level was increased by 637.5%, 240.1%, and 26.8% at 0.001, 0.01, and 0.1 μg/mL, respectively in the BT-DMEM+UT-TI group as compared to the UT-DMEM+UT-TI group. Moreover, the ATP level was increased by 776.2%, 22.9%, 234.2%, and 24.5% at 0.001, 0.01, 0.1, and 1 μg/mL, respectively in the BT-DMEM+BT-TI group as compared with the UT-DMEM+UTTI group. Overall, data suggested that there was significant improvement of ATP level in the Biofield Energy Treated test formulation as well as Biofield Treated DMEM medium. Therefore, the results suggest that the Biofield Energy Treated test formulation and/or the Biofield Energy Treated DMEM medium can be used as energy booster and also it can improve the mental stamina, endurance, and physical strength of the muscles to show the better performance.
