Material Detail

"Construction of an Imitating Nano-motor Driven by Six ATP-binding RNAs of Bacterial Virus phi29" icon

Construction of an Imitating Nano-motor Driven by Six ATP-binding RNAs of Bacterial Virus phi29

A switchable imitating DNA-packaging motor was constructed in the laboratory. The motor is driven by six synthetic ATP-binding pRNA (packaging RNA) molecules that bind to the connector and function in a manner similar to the driving of a bolt with a hex nut. Conformational change and sequential action of the RNA ensure continuous rotation of the motor, with ATP as energy. A 5-um DNA was packaged using this synthetic motor using one ATP to translocate two base pairs of DNA. The DNA-filled capsids were subsequently converted into infectious virus. Direct observation revealed that the motor can tow a bead more than 500 nm. The 3D structures of pRNA/motor complex and the pRNA monomer, dimer and hexamer have been probed by photo affinity crosslinking, chemical modification interference, nuclease... Show More
Rate

Quality

  • User Rating
  • Comments
  • Learning Exercises
  • Bookmark Collections
  • Course ePortfolios
  • Accessibility Info

More about this material

Browse...

Disciplines with similar materials as Construction of an Imitating Nano-motor Driven by Six ATP-binding RNAs of Bacterial Virus phi29

Comments

Log in to participate in the discussions or sign up if you are not already a MERLOT member.