The present in vitro investigation was aimed to explore the effect of Consciousness Energy Healing Treated DMEM medium for oxidative potential using oxidative biomarkers viz. Superoxide Dismutase (SOD) enzyme activity and protection against oxidative stress measured in A549 cells. The test item, DMEM was divided into two parts. First part of the test item received Consciousness Energy Healing Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the Biofield Energy Treated DMEM group, while the second part did not receive any sort of Treatment and defined as the Untreated DMEM (control) group. The cell viability of the test sample using MTT assay showed an increase cell viability by 136.4% in the Biofield Energy Treated DMEM group, suggesting a safe and nontoxic nature of the test items. Protection against oxidative stress was significantly increased by 23.80% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group. Besides, SOD enzyme activity was significantly increased by 40.40% in the Biofield Energy Treated DMEM group compared with the untreated DMEM group. Thus, the overall data suggest that the Biofield Energy Healing Treatment showed a significant improvement of SOD enzyme level along with an improved protection against oxidative damage, which can be used in various human lungs disorders such as asthma, Chronic Obstructive Pulmonary Disease (COPD), lung malignancies and parenchymal lung diseases like idiopathic pulmonary fibrosis and lung granulomatous diseases.