A proprietary formulation was designed that consist of minerals (zinc, magnesium, iron, and copper) and vitamins (pyridoxine HCl, cyanocobalamin, and cholecalciferol). The present study was aimed to evaluate the impact of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats for antioxidant activity. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Additionally, three groups of animals were also received Biofield Energy Healing Treatment per se (day -15). The tissue lipid peroxidation data exhibited that the level of malondialdehyde (MDA) was altered by 20.86%, 12.92%, and 17.31% in the Biofield Energy Treated test formulation from day -15 (G7), Biofield Energy Treatment per se plus Biofield Energy Treated test formulation from day -15 (G8), and Biofield Energy Treatment per se animals plus untreated test formulation (G9) groups, respectively as compared to the untreated test formulation group (G4). Moreover, tissue myeloperoxidase (MPO) level was significantly reduced by 32.46%, 27.49%, 27.49%, and 23.71% in the Biofield Energy Treatment per se to animals from day -15 (G6), G7, G8, and G9 groups, respectively as compared to the G4 group. Antioxidant enzyme like SOD was significantly increased by 26.64%, 28.74%, 22.69%, 36.31%, and 36.11% in the Biofield Energy Treated test formulation (G5), G6, G7, G8, and G9, respectively compared to the disease control group (G2). Additionally, the level of catalase was significantly increased by 156.10%, 101.62%, 66.57%, and 179.17% in the G6, G7, G8, G9 groups, respectively compared to the G4 group. Further, LDH level was significantly reduced by 9.86% in the G9 group compared to the G4 group, while NO level was reduced by 11.42%, 10.76%, and 8.77% in the G5, G6, and G7 groups, respectively as compared to the G2 group. Altogether, results suggested that the Biofield Treated test formulation possess antioxidant potential and could be used to maintain bodies’ oxidative stress, immunity and boost overall health and aging.
