Over the past few decades, Cerebrospinal Fluid (CSF) biomarkers have been reported as the golden standard for the diagnosis of anti-aging disorders and any neurodegenerative diseases. The study objective was to investigate the effect of Consciousness Energy Healing based novel proprietary test formulation for anti-aging activity using identification of Klotho, tau protein along with serotonin and corticosterone levels. Each ingredient of the test formulation was divided into two parts. One portion was defined as the untreated without any Biofield Energy Treatment, while the other portion was marked as the Biofield Energy Healing Treatment based test formulation, which received the Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. All the CSF biomarkers estimation were performed using ELISA assay kit as per manufacturer’s instructions. The level of Klotho was significantly increased by 63% (p≤0.05), 24.5%, 13%, 26.2%, and 41.7% in the experimental test groups viz. G5, G6, G7, G8, and G9, respectively, as compared with the untreated test formulation group (G4). However, the level of corticosterone was found to be decreased by 28.6%, 49.0% (p≤0.05), 18.8%, 51% (p≤0.01), and 62.5% (p≤0.01) in the G5, G6, G7, G8, and G9 groups, respectively, as compared with the G4 group. Similarly, serotonin level was increased by 64.7% and 38% in the Biofield Energy Treated test groups viz. G6 and G8 groups, respectively, as compared with the G4 group. Thus, overall data indicated that the Biofield Energy Healing Treatment would be one of the best approach against aging and its related disorders such as any neuroprotective action, delays aging, improve cognition, age-related diseases, related to aging such as cancer, chronic kidney disease, ataxia, diabetes, and skin atrophy.
