The aim of this experiment was to evaluate the anti-inflammatory potential of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on L-NAME and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats using serum biomarkers. In this experiment various proinflammatory cytokines such as TNF-α, IL-6, IL-18, and IL-1β, along with C - reactive protein (CRP) using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin B9, vitamin B12, and vitamin D3), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one part was denoted as the untreated, while the other part and three groups of animals received Biofield Energy Healing Treatment/Blessing remotely for about 3 minutes by a renowned Spiritual Energy Healer, Mr. Mahendra Kumar Trivedi. The cytokines estimation results showed that the level of tumour necrosis factor-alpha (TNF-α) was reduced by 12.72% in the G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15) group as compared to the disease control (L-NAME + HFD + 0.5% CMC) group (G2). Moreover, the level of interleukin-6 (IL-6) was reduced by 31.75%, 15.96%, 24.18%, and 24.11% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively, as compared to the G2 group. Additionally, the level of interleukin-18 (IL-18) was decreased minimally in the treatment group as compared to the G2 group. The level of IL-1β was decreased by 7.93%, 8.35%, 7.93%, 6.68%, and 6.68% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the untreated test formulation (G4). Besides, the level of CRP was decreased by 33.25%, 25.78%, 15.63%, 42.12%, and 35.40% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested significance antiinflammatory potentials of the Biofield Energy Treated/Blessed test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various inflammatory conditions that might be beneficial various types of cardiovascular disorders. Therefore, the results showed the significant slowdown the inflammation-related cardiovascular disease progression and its complications in the preventive treatment groups (viz. G6, G7, G8, and G9).
