The aim of the present study was to evaluate the vitamin D receptor (VDR) expression after treatment with the Trivedi Effect®- Biofield Energy Treated (Blessed) Test formulation/item (TI). The novel test formulation was composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene, which was tested on various cell lines in DMEM medium using values of relative quantification (RQ) by RT-PCR among different groups. The test formulation constituents were divided into two parts; untreated test formulation (UT), another part received Biofield Energy Healing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. MTT data showed that all the cell lines after treatment with the test formulation in various tested concentrations were found as safe and nontoxic with cell viability ranges from 71% to 181% upto 10 μg/mL. A549 cells showed a significant increased RQ values by 29.9% (UT- DMEM + BT-TI) and 200.2% (BT-DMEM + UT-TI) among the experimental groups as compared with the untreated test formulation. HepG2 cells showed a significant increased RQ value by 10.3% to 233.1% in the UT- DMEM + BT-TI group at 0.01 and 1μg/mL, respectively as compared with the untreated test group. HT-29 cells showed a significant increased RQ value by 34.8% (BT-DMEM + UT-TI group) to 220% (BT-DMEM + BT-TI group); while EA.hy926 cells significantly increased RQ value by 20.9% (BT-DMEM + BT-TI) to 398.8% (UT- DMEM + BT-TI) among the tested group as compared with the untreated test group. C2C12 cells showed a significant increased RQ value by 6.3% to 100% in the BT-DMEM + BT-TI group as compared with the untreated test group. Hence, it can be concluded that the Biofield Energy Treatment (Blessing) may have the potential to upregulate the VDR expression in multiple cell-lines. Overall, the results showed the significant increased VDR expression in terms of increase RQ values in all the tested cell-lines. Hence, it can be concluded that the Biofield Energy Treatment (Blessing) may have the excellent potential to upregulate the VDR expression in multiple cell-lines, and that could help calcium and bone homeostasis, and slowdown of the disease progression rate related to vitamin D3 deficiency.
