The present study was designed to evaluate the VDR (vitamin D receptor) expression after treatment with the Trivedi Effect®- Biofield Energy Treated/Blessed Test formulation (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on various cell lines in DMEM medium using values of relative quantification (RQ) by RTPCR among different groups. The test formulation constituents were divided into two parts; one part defined as the untreated test formulation (UT), another part received Biofield Energy Healing Treatment (BT)/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test formulation was treated with Biofield Energy Healing Treatment/Blessing and was divided as Biofield Energy Treated/Blessed (BT) and untreated (UT) test items. MTT data showed that all the cell lines after treatment with the test formulation in various tested concentrations were found as safe and nontoxic with viability range 72% to 144% up to 1 µg/mL test formulation concentration. In addition, RQ values were calculated in all the cell lines and were compared with the untreated test formulation, which showed significant improved values. The MG-63 cell line showed significant increased RQ values by 140.2% (UT- DMEM + BT-TI) to 817.7% (BT-DMEM + BT-TI) among the experimental groups as compared with the untreated test formulation group. The MDA-MB-231 cell lines showed a significant increased RQ value in all the test formulation groups by 55.3% (BT-DMEM + UT-TI group) to 2308.8% (UT- DMEM + BT-TI group) among the tested groups as compared with the untreated test group. The SH-SY5Y cells were used for the VDR expression in the showed a significant increased RQ value by 15.7% (BT- DMEM + UT-TI group) to 457.7% (BT-DMEM + UT-TI group), while HEK-293 cells significant increased RQ value by 14.6% to 695.8% among the tested group in the UT- DMEM + BT-TI group as compared with the untreated test group. The HaCaT cells showed a significant increased RQ value by 41.9% (UT- DMEM + BT-TI group) to 2123.5% (BT-DMEM + BT-TI group) among the tested groups as compared with the untreated test group. Hence, it can be concluded that the Biofield Energy Treatment may have the potential to modulate vitamin D3 metabolism via VDR expression. Overall, the results showed the significant increased RQ values in all the tested cell line. VDR expression in different cell lines were significantly modulated in MG-63 (Bone), MDA-MB-231 (Breast), SH-SY5Y (Brain), HEK293 (Kidney), and HaCaT (Skin) cell lines. Thus, it can be concluded that the Biofield Energy Treatment/Blessing may have the excellent potential to upregulate the VDR expression in multiple cell-lines, and that could help calcium and bone homeostasis, and slowdown of the disease progression rate related to vitamin D3 deficiency.
