Endothelin-1 and nitric oxide (NO) activities in artery, brain, and kidney tissues were performed in the unpredictable chronic stress (UCS) rat’s model for the evaluation of Consciousness Energy Healing Treated (the Trivedi Effect®) novel proprietary test formulation in male Sprague Dawley (SD) rats using ELISA assay. The test formulation consisted of minerals (Zn, Fe, Cu, Se, Ca, Mg), vitamins (C, E, B6, B12, D3), and nutraceuticals (β-carotene, ginseng, and cannabidiol isolate). The test formulation constituents were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three groups of animals received Biofield Energy Healing Treatment/Blessing by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Artery-tissue elasticity endothelin-1 was decreased by 58.1%, 41.7%, 51.3%, 52%, and 57.1% in the G5 (Biofield Energy Treated Test formulation to the untreated rats), G6 (Biofield Energy Treatment per se to the rats), G7 (15-days pre-treatment of Biofield Energy Treated Test formulation), G8 (15 days pre-treatment of Biofield Energy Treated Test formulation to the Biofield Energy Treatment per se rats), and G9 (untreated test formulation to the Biofield Energy Treatment per se to the rats) groups, respectively as compared with the disease control group (G2). Artery NO level was significantly decreased by 20.9%, 10%, and 30.1% (p≤0.01) in the G5, G6, and G9 groups, respectively as compared with the G2. Similarly, brain endothelin-1 level was significantly decreased by 30.1% (p≤0.001), 12.3%, 23.7% (p≤0.001), 43.4% (p≤0.001), and 43% (p≤0.001) in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. Brain NO was increased by 12.1% and 21% in the G5 and G6 groups, respectively as compared with the G2. Endothelin-1 level in kidney was significantly decreased by 14.2% and 52.4% (p≤0.001) in the G8 and G9 groups, respectively as compared with the G2. Kidney NO level was significantly increased by 25%, 22.9%, 18.3%, 17.3%, and 23.4% in the G5, G6, G7, G8, and G9 groups respectively, as compared with the G4. Overall, the experimental data suggested significance effect of Biofield Energy/Blessing per se along with preventive measure on the animal with respect to various stress-related disorders. Overall, the results showed the significant slowdown the stressrelated disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated/Blessed Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group.
