The study was aimed to investigate the impact of the Trivedi Effect® on high fat diet (HFD) and NG-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced Sprague Dawley rats using various functional organ (aorta, lung, and kidney) biomarkers. The functional biomarkers like endothelin-1 and nitric oxide (NO) were evaluated in all the three tissue homogenate using ELISA. The proprietary test formulation including minerals (selenium, magnesium, zinc, calcium, iron, and copper), vitamins (vitamin B9, ascorbic acid, cyanocobalamin, cholecalciferol, and pyridoxine HCl), cannabidiol isolate, β-carotene, and Panax ginseng extract. The each constituents of the proprietary test formulation were divided into two parts; one was denoted as untreated without any Blessing, while the other part received Biofield Treatment by Mr. Mahendra Kumar Trivedi. The results showed that the level of endothelin-1 in the aorta tissue homogenate was decreased by 52.41%, 50.92%, 42.71%, 32.20%, and 45.66% in the G5, G6, G7, G8, and G9 groups, respectively than disease control (G2) group. The level of NO in the aorta tissue homogenate was increased by 30.77%, 11.02%, 13.96%, and 22.57% in the G5, G6, G7, and G8 than G2. Moreover, the expression of endothelin-1 in the lung tissue homogenate was decreased by 40.95%, 19.69%, 39.94%, 24.09%, and 18.85% in the G5, G6, G7, G8, and G9 groups, respectively than G2. The level of NO in the lung tissue homogenate was increased by 25.29% and 29.16% in the G6 and G7 groups, respectively than G2. Further, the level of endothelin-1 in the kidney tissue homogenate was decreased by 18.65%, 11.27%, 22.84%, 19.44%, and 30.32% in the G5, G6, G7, G8, and G9 groups, respectively than G2. Overall, data suggested a significant improvement of endothelin-1 and nitric oxide activity in different organs. Therefore, the current outcomes could able to envisage a remarkable reduction of cardiovascular complications in the preventive treatment viz. G6, G7, G8, and G9 and might be beneficial various types of cardiovascular disorders and overall health.
