Berberine is a benzylisoquinoline alkaloid found in many medicinal plants such as Berberis vulgaris, Mahonia aquifolium, etc. Although it has enormous therapeutic potential, the bioavailability is very poor (<1%) due to its low solubility and poor intestinal absorption. This study was performed to investigate the impact of the Trivedi Effect® on the structural properties and the isotopic abundance ratio of berberine chloride using LC-MS and GC-MS spectroscopy. Berberine chloride sample was divided into two parts, one part of berberine chloride was considered as a control sample (received no Biofield Energy Treatment), while the second part was treated with the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a famous Biofield Energy Healer, Alice Branton, called a treated sample. The LC-MS spectra of both the samples at retention time (Rt) ~2.1 minutes exhibited the mass of the molecular ion peak at m/z 336.3 [M]+ (calculated for C20H18NO4 +, 336.12). The LC-MS based isotopic abundance ratio of PM+1/PM in the treated berberine was significantly increased by 24.41% compared with the control sample. Thus, 13C, 2H, 15N, and 17O contributions from (C20H18NO4)+ to m/z 337 in the treated sample was significantly increased compared with the control sample. Similarly, the GC-MS based isotopic abundance ratio of PM+1/PM in the treated berberine was significantly increased by 12.97% compared with the control sample. Hence, 13C, 2H, 15N, and 17O contributions from (C20H16NO4)3+ to m/z 335 in the treated sample were significantly increased compared with the control sample. But, the isotopic abundance ratio of PM+2/PM in the treated berberine chloride was significantly decreased by 38.59% compared with the control sample. Hence, 18O contribution from (C20H16NO4)3+ to m/z 336 in the treated sample was significantly decreased compared with the control sample. The isotopic abundance ratios of PM+1/PM and PM+2/PM in the treated berberine chloride were significantly altered compared to the control sample. The altered isotopic abundance ratios (2H/1H or 13C/12C or 15N/14N or 17O/16O or 18O/16O) would influence the intra-atomic bond strength, its physical stability, and alter the rate of reactions in the body. The changes in isotopic abundance might be due to changes in nuclei, possibly through the interference of neutrino particles via the Trivedi Effect®-Consciousness Energy Healing Treatment. The new form of berberine chloride would be more efficacious novel pharmaceutical formulations that might offer better solubility, bioavailability and therapeutic response against diarrhoea, gastroenteritis, bacterial, fungal and other microbial infections, cancer, arrhythmia, diabetes, hyperlipidemia, inflammation in the body, etc.
