Berberine is a benzylisoquinoline alkaloid which has enormous therapeutic potential, but the bioavailability is very poor due to its low solubility and poor intestinal absorption. In this study, the impact of the Trivedi Effect®-Biofield Energy Healing Treatment on the structural properties and the isotopic abundance ratio of berberine chloride was evaluated using advanced spectroscopic methods. Berberine chloride sample was divided into control and treated parts. Only the treated part was received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a famous Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the berberine chloride samples observed at retention time 2 minutes and the molecular ion peak at m/z 336.25 [M]+ (calculated for C20H18NO4+, 336.12). The LC-MS based isotopic abundance ratio of PM+1/PM in the treated berberine chloride was significantly decreased by 18.53% compared with the control sample. Similarly, the GC-MS based isotopic abundance ratio of PM+1/PM in the treated berberine chloride was decreased by 4.69% compared with the control sample. Thus, 13C, 2H, 15N, and 17O contributions from (C20H18NO4)+ to m/z 337 in the treated sample were significantly decreased compared with the control sample. But, the isotopic abundance ratio of PM+2/PM in the treated sample was significantly increased by 11.52% compared with the control sample. Hence, 18O contribution from (C20H16NO4)3+ to m/z 336 in the treated sample was significantly increased compared with the control sample. The isotopic abundance ratios of PM+1/ PM (2H/1H or 13C/12C or 15N/14N or 17O/16O) and PM+2/PM (18O/16O) in the treated berberine chloride were significantly altered compared to the control sample. The changes in isotopic abundance might be due to changes in nuclei possibly through the interference of neutrino particles via the Trivedi Effect®-Consciousness Energy Healing Treatment. The decreased isotopic abundance ratio (PM+1/PM) of the Consciousness Energy Healing Treated berberine chloride may decrease the chemical bond strength, influence its stability, and bioavailability in the body. The new form of berberine chloride would be more efficacious novel pharmaceutical formulations that might offer better solubility, dissolution, absorption, bioavailability and therapeutic response against diarrhoea, gastroenteritis, bacterial and fungal infections, cancer, arrhythmia, diabetes, hyperlipidemia, inflammation, etc.
