The study was aimed to evaluate the gut health protective effect of Biofield Treated/Blessed proprietary test formulation on serum cytokines. Each constituents of the novel test formulation was divided into two parts, one part was denoted as control and the other part was defined as Biofield Energy Treated/Blessed test formulation, which received Biofield Energy Healing Treatment/Blessing (prayer) remotely for about 3 minutes by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The serum was subjected to test the following biomarkers viz. C-reactive protein (CRP), myeloperoxidase (MPO), and pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), IL-10, IL-23, interferon gamma (IFN-γ), and transforming growth factor beta (TGF-β) using ELISA method. CRP level was decreased by 17.12%, 40.13%, 34.79%, 42.28%, and 12.88% in the G5 (Biofield Treated test formulation), G6 (Biofield Energy Treatment per se at day -15), G7 (Biofield Treated test formulation from day-15), G8 (Biofield Treatment/Blessing per se to the animals with Biofield Treated/Blessed test formulation from day -15), and G9 (Biofield Treatment/Blessing per se from day -15 to animals plus untreated test formulation) groups, respectively as compared with the disease control (G2) group. TGF-β level was decreased by 9.51% and 9.99% in the G7 and G8 groups, respectively than untreated test formulation (G4) group. In addition, the level of IL-23 was decreased by 22.86%, 30.61%, 25.69%, 42.15%, and 25.81% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. Further, the level of IL-6 was significantly decreased by 54.4% (p≤0.05), 30.5%, 20%, and 48% (p≤0.05) in the G5, G6, G7, and G8 groups, respectively with respect to the G4 group. There was an alteration of TNF-α compared to G2. Besides, IL-10 level was increased by 138.63%, 169.26%, 106.07%, 102.79%, and 81.55% in G5, G6, G7, G8, and G9 groups, respectively as compared with the G2 group. However, the serum MPO level was significantly decreased by 21.33% (p≤0.05), 15.42%, 22.61% (p≤0.05), 13.21%, and 26.25% (p≤0.05) in G5, G6, G7, G8, and G9 respectively, than G2 group. The Trivedi Effect®-Consciousness Energy Healing/Blessing (prayer) would be the best alternative approach for treating gut inflammatory disorders. Thus, the results showed the significant retard the inflammation-related to gastrointestinal diseases (Crohn’s disease, ulcerative colitis, along with microbial, parasitic, and viral inflammatory disorders), and its complications in the preventive treatment groups (viz. G6, G7, G8, and G9).
