The study was planned to assess the antiaging, antistress, and neuroprotective potential of the Biofield Energy Treated/Blessed novel Proprietary Test Formulation and Biofield Energy Treatment per se to the animals on NG-nitro-L-arginine methyl ester A hydrochloride (L-NAME) and high fat diet (HFD)-induced cardiovascular model in Sprague Dawley rats using various functional biomarkers in cerebrospinal fluids (CSF). The functional biomarkers like klotho protein, dopamine, corticosterone, tao protein, and norepinephrine in CSF using ELISA assay for the assessment of antiaging, antistress, and neuroprotective activities. The test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin B9, cholecalciferol, and cyanocobalamin), cannabidiol isolate, Panax ginseng extract, and β-carotene. The ingredients of the test formulation were divided into two parts; one part was denoted as the untreated and other part of the test formulation and three groups of animals received Blessing remotely for about 3 minutes by Mr. Mahendra Kumar Trivedi, a renowned Biofield Energy Healer. Klotho protein was significantly increased by 12.24%, 33.89%, 22.82%, 31.26%, and 21.66% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G7 (L-NAME + HFD + the Biofield Energy Treated/Blessed test formulation from day -15), G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively, as compared to the (L-NAME + HFD + untreated test formulation) group (G4). Moreover, the level of dopamine was increased by 18.47% in the G6 group as compared to the G2 group. Corticosterone was significantly decreased by 95.96%, 93.61%, 94.68%, 97.96%, and 93.04% in the G5, G6, G7, G8, and G9 groups, respectively than G2 group. Additionally, the level of tao protein was increased by 12.16% in the G6 group as compared to the G2 group. Further, the level of norepinephrine was increased by 10% in the G7 group as compared to the G4 group. Overall, the data suggested a significant antiaging activity by increasing the levels of klotho protein, antistress activity by reducing the level of corticosterone, and neuroprotective activity by increasing the levels of dopamine, tao protein, and norepinephrine in CSF of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animals that might be beneficial various types of cardiovascular disorders. Therefore, the results showed the significant slowdown the oxidative stress-related cardiovascular disease progression and its complications and/or symptoms in the preventive treatment group per se and/or Biofield Energy Treated/Blessed Test formulation groups (viz. G6, G7, G8, and G9).
