Metronidazole is a well-known antibacterial and antiprotozoal antibiotic. In this study, the impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of metronidazole were evaluated using LC-MS and GC-MS spectroscopy. The test sample (metronidazole) was divided into two control and treated parts. Only the treated sample received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the samples of metronidazole at the retention time 2.61 minutes exhibited the mass of the protonated molecular ion peak at m/z 172 [M+H]+, but the peak area of the treated metronidazole was significantly decreased by 13.1% compared to the control sample. The LC-MS and GC-MS based isotopic abundance ratios of PM+1/PM in the treated metronidazole was significantly increased by 46.43% and 13.52%, respectively compared with the control sample. Thus, 13C, 2H, 15N, and 17O contributions from (C6H10N3O3)+ to m/z 173 in the treated sample were significantly increased compared with the control sample. The increased isotopic composition in the molecular level of the treated metronidazole might be due to the alteration in neutron to proton ratio in the nucleus possibly through the interference of neutrino via the Trivedi Effect®. The isotopic abundance ratios 2H/1H, 13C/12C, 17O/16O, and 15N/14N would highly influence the atomic bond vibration of treated metronidazole. The increased isotopic abundance ratio of the treated metronidazole would stronger the chemical bond and increase the stability in the body. The treated metronidazole would be very useful to design more efficacious pharmaceutical formulations that might offer better therapeutic response againstbacterial vaginosis, giardiasis, trichomoniasis, pseudomembranous colitis, pelvic inflammatory disease;liver, skin, brain, and respiratory tract infections; aspiration pneumonia, rosacea, intra-abdominal infections, lung abscess, fungating wounds, periodontitis, amoebiasis, oral infections, etc.
