Metronidazole is a common antimicrobial antibiotic, which is used in the treatment of bacterial vaginosis, giardiasis, trichomoniasis, pseudomembranous colitis, etc. This study was performed to evaluate the impact of the Trivedi Effect® on the structural properties, and the isotopic abundance ratio of metronidazole evaluated using GC-MS analytical techniques. The metronidazole sample was divided into control and treated parts. Only the treated part received the Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Gopal Nayak. The GC-MS spectra of both the samples of metronidazole at the retention time 16.4 minutes exhibited the molecular ion peak at m/z 171 [M]+ (calculated for C6H9N3O3+, 171.06). The peak area of the Biofield Energy Treated metronidazole was significantly increased by 166.83% compared to the control sample. The isotopic abundance ratio of PM+1/PM in the treated metronidazole was significantly decreased by 19.72% compared with the control sample. Thus, 13C, 2H, 15N, and 17O contributions from (C6H9N3O3)+ to m/z 172 in the treated sample were significantly decreased compared with the control sample. The decreased isotopic composition of the treated metronidazole might be the cause of interference of neutrino via the Trivedi Effect®. The isotopic abundance ratios 2H/1H, 13C/12C, 17O/16O, and 15N/14N would highly influence the atomic bond vibration of treated metronidazole. The decreased isotopic abundance ratio would weaken the chemical bond and increase the solubility of the treated metronidazole. The new form of Biofield Energy Treated metronidazole would be very useful to design more efficacious pharmaceutical formulations that might offer a better therapeutic response against bacterial vaginosis, pseudomembranous colitis, trichomoniasis, giardiasis, pelvic inflammatory disease, brain, and respiratory tract infection, etc.
