Material Detail

Virtual Bacterial Identification Lab

Virtual Bacterial Identification Lab

A virtual lab where you carry out the sequential steps to isolate bacterai, purify DNA, PCR amplify the DNA, sequence the 16S ribosomal region and then use the sequence for a blast search of Genbank to finally identify the unknown bacterium.
Rate

Quality

More about this material

Comments

Log in to participate in the discussions or sign up if you are not already a MERLOT member.
Sunita Singh
Sunita Singh (Faculty)
3 years ago
Very nice instruction maerial
Christopher Kitts
Christopher Kitts (Faculty)
4 years ago
Page is now missing
Time spent reviewing site: N/a
alan elkins
alan elkins (Faculty)
15 years ago
I navigated the virtual lab and was quite impressed. Normally I do care for
virtual labs but this site offers procedures that most students could never
experience otherwise.

Technical Remarks:

The Bacterial ID Lab incorporates all of the processes involved in DNA
sequencing from sample preparation to final analysis. It allows a student with
limited lab skills and no access to expensive equipment to experience a ?state
of the art? procedure.
It demonstrates the tools and technology the make gene sequencing possible and
removes some of the mystery accompanying so complex a process. The non-biology
major will gain a better understanding of the technique and its application. The
site is arranged in six stages that the student can easily navigate. The
student can visit and revisit each procedure. Instructions and explanations are
provided so that the student understands each step.
Used in course? Yes