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Nanoscale functionalities for biopharmaceutical drug delivery

Nanoscale functionalities for biopharmaceutical drug delivery

This video was recorded at SLONANO conference, Ljubljana 2007. One of our recent research challenges has been exploitation of the active principle of protein aggregation, involving metal coordination of specifically designed protein analogues. Specifically designed analogues of tumour necrosis factor alpha (TNF-alpha) rich in histidines served as model proteins. LK801 is a TNF-alpha analogue with double histidine mutation (Glu107HisGly108HisTNF-alpha) in the tip region of the bell-shaped molecule. Due to the symmetrical trimeric structure, histidine residues in the tip region form an almost planar cluster of six well accessible histidines resulting in strong binding to Immobilized Metal Affinity Chromatography (IMAC), which was used for efficient single step purification. IMAC was also used... Show More
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