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Alkaline phosphatase (ALP) plays a vital role in the endometrium development, maturation, endocrine function, and osteoblastic differentiation. The present investigation explained the role of Consciousness Energy Healing based test item (DMEM medium) on ALP in Ishikawa cells. After the division of the test item into two parts; first, part received Biofield Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and was labeled as the Biofield Treated DMEM, while the second part did not receive any treatment, and denoted as the untreated DMEM. MTT data showed 142.56% viable cells in the Biofield Treated test item, which indicated that the test item was found as safe and non-toxic. Besides, the estrogenic potential concerning ALP level was significantly (p≤0.001) increased by 65.18% in the Biofield Treated DMEM group than untreated DMEM. Thus, the experimental data suggested that the Biofield Energy Treated DMEM would play a significant role for the growth of Ishikawa cells along with an improved ALP level, which play a vital role in the promotion and maintenance of estrogen level and can be used against various estrogenic disorders viz. endometritis, hypophosphatasia, osteoporosis, infertility, severe anemia, malnutrition, fetal defects, early pregnancy loss, and preterm birth.

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