Pyridoxine (vitamin B6) is an essential water-soluble vitamin widely used in nutraceutical and pharmaceutical formulations for the prevention and treatment of vitamin B6 deficiency and other diseases. The objective of the study was to explore the impact of the Trivedi Effect®-Consciousness Energy Treatment on the isotopic abundance ratios and structural properties of pyridoxine hydrochloride using LC-MS, GC-MS, and NMR analytical techniques. The pyridoxine sample was equally divided into control/ untreated and Biofield Energy Treated pyridoxine. The treated pyridoxine sample received Biofield Energy Treatment (the Trivedi Effect®) remotely for ~3 minutes by Mr. Mahendra Kumar Trivedi, who was located in the USA, while the test samples were located in the research laboratory in India. The treated sample was designated as the Biofield Energy Treated sample. The LC-ESI-MS analysis of both of the pyridoxine samples showed the protonated parent ion mass at m/z 170 (calcd for C8H12NO3+, 170.08) at retention times (Rt) 2.41 minutes. But, the relative peak intensities of the treated pyridoxine were significantly improved compared to the control sample. The isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O) and PM+2/PM (18O/16O) were significantly increased by 11.92% and 153.85%, respectively in the treated pyridoxine compared to the control sample. Thus, 2H, 13C, 2H, 15N, 17O contributions from C8H12NO3+ to the isotopic m/z 171 and 18O contribution to the isotopic m/z 172 was significantly increased in the treated sample compared with the control sample. The GC chromatographic peak area% and GC-MS mass peak intensity of the treated sample was significantly increased by 15.88% and 32.26%, respectively compared to the control sample. The proton and carbon signals for CH3, CH2, NH2, CH, =C=, and C-OH groups in the 1H and 13C NMR spectra of the control and treated samples were similar. The increased mass peak intensities, peak area%, and isotopic abundance ratios in the Biofield Energy Treated pyridoxine might be the impact of the Trivedi Effect®-Consciousness Energy Treatment via the possible mediation of neutrinos, which further lead to the change in the kinetic isotope effects of the treated pyridoxine. Thus, the Trivedi Effect® Treated pyridoxine might be advantageous for designing better nutraceutical, dietary supplements and/or pharmaceutical formulations which might provide better therapeutic response against vitamin B6 deficiency, hereditary sideroblastic anemia, premenstrual syndrome, cardiovascular disease, pyridoxine-dependency seizures, febrile seizures, pulmonary tuberculosis, metabolic disorders, Alzheimer’s disease, cancer, hyperhomocysteinemia, anxiety, asthma, depression, attention deficit hyperactivity disorder, dysmenorrhea, akathisia, angioplasty, birth outcomes, cognitive function, hyperkinetic cerebral dysfunction syndrome, carpal tunnel syndrome, hypertension, immune system function, breast pain, pregnancy-induced nausea and vomiting, lactation suppression, McArdle’s disease, osteoporosis, Tardive dyskinesia, autism, stroke recurrence, etc.
