Vitamin D3 (cholecalciferol) has multiple effects which regulate the functions of muscles, brain, lungs, liver, kidneys, heart, immune system, pancreas, large and small intestines. The aim of the study was to investigate the impact of The Trivedi Effect®-Consciousness Energy Healing Treatment on the isotopic abundance ratios (PM+1/PM and PM+2/PM) and structural properties of vitamin D3 using LC-MS, GC-MS, and NMR spectroscopy. Vitamin D3 was divided into two parts, one part of the sample was termed as untreated (control) sample, while the other part of the sample received The Trivedi Effect®-Biofield Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and termed as Biofield Energy Treated sample. The LC-ESI-MS analysis of both the samples showed the mass of protonated ion at m/z 385.3 (calcd for C27H45O+, 385.35) at the retention time (Rt) 20.7 minutes. The liquid chromatographic peak area of the treated sample was significantly improved by 16.00% compared to the control sample. Similarly, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) was very significantly increased by 470.88% in the treated sample compared to the control sample. But, the isotopic abundance ratio of PM+2/PM(18O/16O) in the treated sample also significantly decreased by 41.81% compared to the control sample. Therefore, the 2H, 13C, and 17O contributions from C27H45O+ to m/z 386 were significantly increased, whereas 18O contribution from C18H18O6+ to m/z 387 was decreased in the treated sample compared with the control sample. The GC-MS analysis showed that the parent molecular mass peak intensities (m/z 384.4) in the treated sample at Rt 23.04 and 23.65 minutes were significantly increased by 6.11% and 9.62%, respectively compared with the control sample. The proton and carbon signals for CH3, CH2, CH, C-OH, and =C= groups in the 1H and 13C NMR spectra of the control and treated samples were similar. The altered isotopic abundance ratios and mass peak intensities might be due to energy produced by The Trivedi Effect®-Consciousness Energy Healing Treatment via the possible mediation of neutrinos, which further lead to the change in the kinetic isotope effects and physicochemical properties of the treated sample. Thus, The Trivedi Effect® Treated vitamin D3 would be advantageous for designing better nutraceutical and pharmaceutical formulations which might provide better therapeutic response against vitamin D deficiency, rickets, osteoporosis, arthritis, multiple sclerosis, diabetes, cancer, cardiovascular diseases, mental disorders, infections, stress, aging, glucose intolerance, multiple sclerosis, Parkinson’s and Alzheimer’s diseases, dementia, etc.
