Magnesium is a second most abundant mineral in the human body and, which is required for in more than 300 enzymatic reactions, normal functioning of body organs, etc. The main objective of the current study was to investigate the impact of The Trivedi Effect®- Consciousness Energy Healing Treatment on magnesium gluconate for the change in the isotopic abundance ratio (PM+1/PM and PM+2/PM) along with the structural properties using LC-MS and NMR spectroscopy. Magnesium gluconate sample was divided into two parts – one part termed as control, while the other part was treated with The Trivedi Effect®- Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and termed as Biofield Energy Treated sample. The LC-MS analysis of the control and Biofield Energy Treated samples exhibited the presence of the molecular mass of magnesium gluconate adduct with hydrogen ion at m/z 415 (calcd for C12H23MgO14+, 415.09) at the retention time of 1.67 minutes and almost similar fragmentation pattern. But, the relative peak intensities of the fragmented ions of the Biofield Energy Treated sample were significantly changed compared with the control sample. The proton and carbon signals for CH, CH2, OH, and CO groups in the 1H and 13C NMR spectra of the treated sample were almost close compared with the control sample. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated magnesium gluconate was significantly decreased by 60.41% compared to the control sample. Therefore, the 13C, 2H, 17O, and 25Mg contributions from (C12H23MgO14)+ to m/z 416 in the treated sample was significantly decreased compared to the control sample. Similarly, the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) in the treated sample was significantly decreased by 79.33% compared to the control sample. Briefly, the 18O and 26Mg contributions from (C12H23MgO14)+ to m/z 417 in the treated magnesium gluconate significantly decreased compared with the control sample. Thus, The Trivedi Effect®-Consciousness Energy Healing Treated magnesium gluconate might be supportive to design the novel potent enzyme inhibitors and better pharmaceutical and nutraceutical formulations through its changed intrinsic properties. This Biofield Energy Treated magnesium gluconate could prove better therapeutic response against various diseases such as diabetes mellitus, aging, allergy, immunological disorders, inflammatory diseases, and other chronic infections.
