Vitamin D3 (cholecalciferol) is a fat-soluble vitamin, which widely used for the prevention and treatment of vitamin D deficiency disorders. This study was designed to investigate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment on the structural properties and the isotopic abundance ratio of cholecalciferol using LC-MS and GC-MS spectroscopy. Cholecalciferol sample was divided into two parts, one part of cholecalciferol was considered as a control sample (no Biofield Energy Treatment was provided), while the second part was treated with The Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Alice Branton and termed as the treated sample. The LC-MS spectra of both the samples at retention time (Rt) ~21.5 minutes exhibited the mass of the molecular ion peak at m/z 385.25 (calcd for C27H45O+, 385.35). The LC-MS based isotopic abundance ratio of PM+1/PM in the treated cholecalciferol was increased by 1.9% compared with the control sample. The control and treated cholecalciferol showed the presence of the chromatographic peak at the retention time of ~22.7min in the GC-MS chromatograms. The GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated cholecalciferol was significantly increased by 1.99% and 15.69%, respectively compared with the control sample. Hence, 13C, 2H, 17O and 18O contributions from C27H44O+ to m/z 386 and 387 in the Biofield Energy Treated sample were significantly increased compared with the control sample. The isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 17O/16O) and PM+2/PM (18O/16O) in the Biofield Energy Treated cholecalciferol were significantly increased compared to the control sample. It can be assumed that the changes in isotopic abundance and mass peak intensities could be due to changes in nuclei possibly through the interference of neutrino particles via The Trivedi Effect® - Consciousness Energy Healing Treatment. The new form of cholecalciferol would be better designing novel pharmaceutical formulations that might offer better therapeutic response against rickets, osteoporosis, arthritis, multiple sclerosis, cancer, diabetes mellitus, mental disorders, cardiovascular diseases, infections, cognitive impairment in older adults, Parkinson’s and Alzheimer’s diseases, dementia, glucose intolerance, multiple sclerosis, etc.
